Improved determination of total serum lipids by the sulfo-phospho-vanillin reaction.

to the compounds tested, for at least an hour under the described conditions, but biologically reliable and physiologically representative results probably can be obtained for a much longer time when the reasons for tubular damage have been identified and corrected. Aside from the fact that different metabolites were studied, our experimental arrangement differed from that used in studies of the isolated perfused kidney [e.g., (3)] or in cross-perfusion studies [e.g., (4)]. We selectively catheterized one renal artery and vein and left the rest of the body circulation undisturbed. Moreover, the various instrumentation also permitted more precise monitoring and (or) control of intrarenal resistance, flow-rate, pulsatile pressure, temperature, PH, F02, and Pco, than perhaps is typically the case for such experiments. Despite this, it is instructive that the subtle homeostatic requirements of the kidney were not met in most cases. When the contralateral kidney was removed three weeks postoperatively, subsequent survival was in most cases brief, with uremia being the cause of death.